The single-grain method: adding TEM to the equation

Autor(en)
Silvia Ulrich, Friđgeir Grímsson
Abstrakt

An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The ‘loss’ of pollen grains and pollen that is ‘difficult to locate’ within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.

Organisation(en)
Department für Botanik und Biodiversitätsforschung
Journal
Grana
Band
59
Seiten
44-57
ISSN
0017-3134
DOI
https://doi.org/10.1080/00173134.2019.1666915
Publikationsdatum
01-2019
Peer-reviewed
Ja
ÖFOS 2012
105117 Paläobotanik, 106008 Botanik, 106049 Ultrastrukturforschung
Schlagwörter
ASJC Scopus Sachgebiete
Ecology, Evolution, Behavior and Systematics, Plant Science
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/the-singlegrain-method-adding-tem-to-the-equation(e2db0f40-9e04-4f07-9448-42cef866d666).html