Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system

Autor(en)
Lekha T. Pazhamala, Palak Chaturvedi, Prasad Bajaj, Sandhya Srikanth, Arindam Ghatak, Annapurna Chitikineni, Anke Bellaire, Anupama Hingane, C.V. Sameer Kumar, K.B. Saxena, Wolfram Weckwerth, Rachit K. Saxena, Rajeev K. Varshney
Abstrakt

Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid regions of Asia and Africa. It is a rich source of protein and capable of alleviating malnutrition, improving soil health and the livelihoods of small-holder farmers. Hybrid breeding has provided remarkable improvements for pigeonpea productivity, but owing to a tedious and costly seed production system, an alternative two-line hybrid technology is being explored. In this regard, an environment-sensitive male sterile line has been characterized as a thermosensitive male sterile line in pigeonpea precisely responding to day temperature. The male sterile and fertile anthers from five developmental stages were studied by integrating transcriptomics, proteomics and metabolomics supported by precise phenotyping and scanning electron microscopic study. Spatio-temporal analysis of anther transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers that play a critical role in normal cell wall morphogenesis and tapetal cell development. The male fertility to sterility transition was mainly due to a perturbation in auxin homeostasis, leading to impaired cell wall modification and sugar transport. Limited nutrient utilization thus leads to microspore starvation in response to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism that underpins fertility transition responses thereby providing a process-oriented two-line hybrid breeding framework for pigeonpea.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie, Department für Botanik und Biodiversitätsforschung, Großgeräteeinrichtung für Massenspektrometrie in den Lebenswissenschaften, Forschungsplattform Vienna Metabolomics Center
Externe Organisation(en)
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) India, Universität Wien, University of Western Australia
Journal
The Plant Genome
Band
13
DOI
https://doi.org/10.1002/tpg2.20028
Publikationsdatum
07-2020
Peer-reviewed
Ja
ÖFOS 2012
106044 Systembiologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/multiomics-approach-unravels-fertility-transition-in-a-pigeonpea-line-for-a-twoline-hybrid-system(fc0e9324-86f7-4c75-87f3-f0c9524b7baa).html