Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system

Autor(en)

Lekha T. Pazhamala, Palak Chaturvedi, Prasad Bajaj, Sandhya Srikanth, Arindam Ghatak, Annapurna Chitikineni, Anke Bellaire, Anupama Hingane, C.V. Sameer Kumar, K.B. Saxena, Wolfram Weckwerth, Rachit K. Saxena, Rajeev K. Varshney

Abstrakt

Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid regions of Asia and Africa. It is a rich source of protein and capable of alleviating malnutrition, improving soil health and the livelihoods of small-holder farmers. Hybrid breeding has provided remarkable improvements for pigeonpea productivity, but owing to a tedious and costly seed production system, an alternative two-line hybrid technology is being explored. In this regard, an environment-sensitive male sterile line has been characterized as a thermosensitive male sterile line in pigeonpea precisely responding to day temperature. The male sterile and fertile anthers from five developmental stages were studied by integrating transcriptomics, proteomics and metabolomics supported by precise phenotyping and scanning electron microscopic study. Spatio-temporal analysis of anther transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers that play a critical role in normal cell wall morphogenesis and tapetal cell development. The male fertility to sterility transition was mainly due to a perturbation in auxin homeostasis, leading to impaired cell wall modification and sugar transport. Limited nutrient utilization thus leads to microspore starvation in response to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism that underpins fertility transition responses thereby providing a process-oriented two-line hybrid breeding framework for pigeonpea.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie, Department für Botanik und Biodiversitätsforschung, Forschungsplattform Vienna Metabolomics Center

Externe Organisation(en)

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) India, University of Western Australia

Journal

The Plant Genome

Band

13

DOI

https://doi.org/10.1002/tpg2.20028

Publikationsdatum

07-2020

Peer-reviewed

Ja

ÖFOS 2012

106044 Systembiologie

Schlagwörter

ASJC Scopus Sachgebiete

Genetics, Agronomy and Crop Science, Plant Science

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/multiomics-approach-unravels-fertility-transition-in-a-pigeonpea-line-for-a-twoline-hybrid-system(fc0e9324-86f7-4c75-87f3-f0c9524b7baa).html