The short life of the Hoyle organ of Sepia officinalis: formation, differentiation and degradation by programmed cell death

Author(s)
Norbert Cyran, Anna Palumbo, Waltraud Klepal, Erica A. G. Vidal, Yannick Staedler, Jürg Schönenberger, Janek von Byern
Abstract

Cephalopods encapsulate their eggs in protective egg envelopes. To hatch from this enclosure, most cephalopod embryos release egg shell-digesting choriolytic enzymes produced by the Hoyle organ (HO). After hatching, this gland becomes inactive and rapidly degrades by programmed cell death. We aim to characterize morphologically the development, maturation and degradation of the gland throughout embryonic and first juvenile stages in Sepia officinalis. Special focus is laid on cell death mechanisms and the presence of nitric oxide synthase during gland degradation. Hatching enzyme has been examined in view of metallic contents, commonly amplifying enzyme effectiveness. HO gland cells are first visualized at embryonic stage 23; secretion is observed from stage 27 onwards. Degradation of the HO occurs after hatching within two days by the rarely observed autophagic process, recognized for the first time in cephalopods. Nitric oxide synthase immunopositivity was not found in the HO cells after hatching, suggesting a possible NO role in cell death signalling. Although the HO ‘life course’ chronology in S. officinalis is similar to other cephalopods, gland degradation occurs by autophagy instead of necrosis. Eggs that combine a large perivitelline space and multi-layered integument seem to require a more complex and large gland system.

Organisation(s)
Core Facility of Cell Imaging and Ultrastructure Research, Department of Botany and Biodiversity Research
External organisation(s)
Stazione Zoologica Anton Dohrn, Federal University of Parana
Journal
Hydrobiologia: the international journal on limnology and marine sciences
Volume
808
Pages
35-55
No. of pages
21
ISSN
0018-8158
DOI
https://doi.org/10.1007/s10750-017-3291-3
Publication date
2018
Peer reviewed
Yes
Austrian Fields of Science 2012
106010 Developmental biology, 106052 Cell biology, 106049 Ultrastructure research, 106012 Evolutionary research
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/the-short-life-of-the-hoyle-organ-of-sepia-officinalis-formation-differentiation-and-degradation-by-programmed-cell-death(7bb37229-bdd2-4b35-a0ba-1edce5ccc03b).html