The single-grain method: adding TEM to the equation

Author(s)
Silvia Ulrich, Friđgeir Grímsson
Abstract

An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The ‘loss’ of pollen grains and pollen that is ‘difficult to locate’ within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.

Organisation(s)
Department of Botany and Biodiversity Research
Journal
Grana
Volume
59
Pages
44-57
ISSN
0017-3134
DOI
https://doi.org/10.1080/00173134.2019.1666915
Publication date
01-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
105117 Palaeobotany, 106008 Botany, 106049 Ultrastructure research
Keywords
ASJC Scopus subject areas
Ecology, Evolution, Behavior and Systematics, Plant Science
Portal url
https://ucris.univie.ac.at/portal/en/publications/the-singlegrain-method-adding-tem-to-the-equation(e2db0f40-9e04-4f07-9448-42cef866d666).html