Bacterial diversity in arboreal ant nesting spaces is linked to colony developmental stage

Author(s)
Maximilian Nepel, Veronika E. Mayer, Veronica Barrajon-Santos, Dagmar Woebken
Abstract

The omnipresence of ants is commonly attributed to their eusocial organization and division of labor, however, bacteria in their nests may facilitate their success. Like many other arboreal ants living in plant-provided cavities, Azteca ants form dark-colored “patches” in their nesting space inside Cecropia host plants. These patches are inhabited by bacteria, fungi and nematodes and appear to be essential for ant colony development. Yet, detailed knowledge of the microbial community composition and its consistency throughout the life cycle of ant colonies was lacking. Amplicon sequencing of the microbial 16S rRNA genes in patches from established ant colonies reveals a highly diverse, ant species-specific bacterial community and little variation within an individual ant colony, with Burkholderiales, Rhizobiales and Chitinophagales being most abundant. In contrast, bacterial communities of early ant colony stages show low alpha diversity and no ant species-specific community composition. We suggest a substrate-caused bottleneck after vertical transmission of the bacterial patch community from mother to daughter colonies. The subsequent ecological succession is driven by environmental parameters and influenced by ant behavior. Our study provides key information for future investigations determining the functions of these bacteria, which is essential to understand the ubiquity of such patches among arboreal ants.

Organisation(s)
Department of Botany and Biodiversity Research, Department of Microbiology and Ecosystem Science
Journal
Communications Biology
Volume
6
ISSN
2399-3642
DOI
https://doi.org/10.1038/s42003-023-05577-5
Publication date
12-2023
Peer reviewed
Yes
Austrian Fields of Science 2012
106008 Botany, 106012 Evolutionary research, 106042 Systematic botany
ASJC Scopus subject areas
General Agricultural and Biological Sciences, General Biochemistry,Genetics and Molecular Biology, Medicine (miscellaneous)
Portal url
https://ucrisportal.univie.ac.at/en/publications/223d5335-0ade-4c8c-a75b-9e0934ee1a8a