The endexine: a frequently overlooked pollen wall layer and a simple method for detection

Author(s)
Martina Weber, Silvia Ulrich
Abstract

For the detection of pollen wall layers, the use of different staining methods for one and the same species is highly recommended. The usage of standard transmission electron microscopy (TEM) staining methods showed that the ektexine-layers have always the same contrast behaviour, while the endexine changes its electron opaqueness depending on the method used. However, the endexine can often not be discriminated from the other wall layers. A simple method to detect the endexine is the use of potassium permanganate, which stains the layer electron dense, producing a distinct contrast.

Organisation(s)
Journal
Grana: an international journal of palynology and aerobiology with world pollen and spore flora
Volume
49
Pages
83-90
No. of pages
8
ISSN
0017-3134
DOI
https://doi.org/10.1080/00173131003743949
Publication date
2010
Peer reviewed
Yes
Austrian Fields of Science 2012
1060 Biology
Portal url
https://ucrisportal.univie.ac.at/en/publications/3b62f298-876e-4c6b-9ec8-55dea015e509