Emergence of a floral colour polymorphism by pollinator-mediated overdominance

Author(s)
Roman T. Kellenberger, Kelsey J. R. P. Byers, Rita M. De Brito Francisco, Yannick M. Staedler, Amy M. LaFountain, Jürg Schönenberger, Florian P. Schiestl, Philipp M. Schlüter
Abstract

Maintenance of polymorphism by overdominance (heterozygote advantage) is a fundamental concept in evolutionary biology. In most examples known in nature, overdominance is a result of homozygotes suffering from deleterious effects. Here we show that overdominance maintains a non-deleterious polymorphism with black, red and white floral morphs in the Alpine orchid Gymnadenia rhellicani. Phenotypic, metabolomic and transcriptomic analyses reveal that the morphs differ solely in cyanidin pigments, which are linked to differential expression of an anthocyanidin synthase (ANS) gene. This expression difference is caused by a premature stop codon in an ANS-regulating R2R3-MYB transcription factor, which is heterozygous in the red colour morph. Furthermore, field observations show that bee and fly pollinators have opposite colour preferences; this results in higher fitness (seed set) of the heterozygous morph without deleterious effects in either homozygous morph. Together, these findings demonstrate that genuine overdominance exists in nature.

Organisation(s)
Department of Botany and Biodiversity Research
External organisation(s)
Universität Zürich (UZH), University of Connecticut, Universität Hohenheim
Journal
Nature Communications
Volume
10
No. of pages
11
ISSN
2041-1723
DOI
https://doi.org/10.1038/s41467-018-07936-x
Publication date
2019
Peer reviewed
Yes
Austrian Fields of Science 2012
106008 Botany, 106012 Evolutionary research, 106042 Systematic botany
Keywords
ASJC Scopus subject areas
General Physics and Astronomy, General Chemistry, General Biochemistry,Genetics and Molecular Biology
Portal url
https://ucrisportal.univie.ac.at/en/publications/765e4342-23f0-4958-848f-c0581cc38d3f